Your browser doesn't support javascript.
loading
An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk
Taheri, Parinaz; Samadi, Nasrin; Ehsani, Mohammad Reza; Khoshayand, Mohammad Reza; Jamalifar, Hossein.
Afiliação
  • Taheri, Parinaz; Islamic Azad University. Faculty of Agriculture and Natural Resources, Science and Research Branch. Department of Food Science and Technology. Tehran. IR
  • Samadi, Nasrin; Tehran University of Medical Sciences. Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center. Department of Drug and Food Control. Tehran. IR
  • Ehsani, Mohammad Reza; Islamic Azad University. Faculty of Agriculture and Natural Resources, Science and Research Branch. Department of Food Science and Technology. Tehran. IR
  • Khoshayand, Mohammad Reza; Tehran University of Medical Sciences. Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center. Department of Drug and Food Control. Tehran. IR
  • Jamalifar, Hossein; Tehran University of Medical Sciences. Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center. Department of Drug and Food Control. Tehran. IR
Braz. j. microbiol ; 43(4): 1452-1462, Oct.-Dec. 2012. graf, tab
Artigo em Inglês | LILACS | ID: lil-665832
Biblioteca responsável: BR32.1
ABSTRACT
A bacteriocin-like inhibitory substance producing Lactococcus lactis subsp lactis strain, ST1, isolated from goat milk of Iranian origin and with broad spectrum of activity and desirable technical properties was used for evaluating some futures of bacteriocin inhibitory activity. Cell growth and bacteriocin production studies were carried out in MRS medium incubated statically under uncontrolled pH condition. The antibacterial activity presented a primary metabolite pattern and showed a rapid decrease at the stationary phase. Microaerobiosis and capnophily growth conditions resulted in higher bacteriocin production while aerobiosis showed negative effect on both cell growth and bacteriocin production. Bacteriocin production, on the other hand, was favored in MRS broth (pH; 6.5) inoculated with 0.1 ml l-1 fresh culture when incubation was carried out at 30 °C. This indicated that the conditions resulted in higher levels of growth were frequently favoring bacteriocin production by ST1 as well. Decrease in activity, at the stationary growth phase, was much pronounced in favored growth condition. Nutrient depletion, deferent effect of low pH on bacteriocin production and/or protein degradation seemed more responsible for this phenomenon. The study also provided further data on new method for bacteriocin release from the cell wall of producer. It was clearly shown that both heating and ultrasound shock for 5 min at pH 2 could increase bacteriocin activity significantly. The release was more pronounced in the presence of 0.5% Tween80.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Saúde e Bem-Estar Problema de saúde: Meta 3.9: Reduzir o número de mortes por produtos químicos perigosos e contaminação do ar e água do solo Base de dados: LILACS Assunto principal: Bacteriocinas / Ultrassom / Plasmídeos de Bacteriocinas / Leite / Lactobacillus / Antibacterianos Tipo de estudo: Estudo de avaliação Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2012 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: Islamic Azad University/IR / Tehran University of Medical Sciences/IR

Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Saúde e Bem-Estar Problema de saúde: Meta 3.9: Reduzir o número de mortes por produtos químicos perigosos e contaminação do ar e água do solo Base de dados: LILACS Assunto principal: Bacteriocinas / Ultrassom / Plasmídeos de Bacteriocinas / Leite / Lactobacillus / Antibacterianos Tipo de estudo: Estudo de avaliação Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2012 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: Islamic Azad University/IR / Tehran University of Medical Sciences/IR
...