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Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP
Geum-Hwa, Lee; Hyung-Ryong, Kim; Sang-Yong, Han; Bidur, Bhandary; Do-Sung, Kim; Min-Gul, Kim; Byung-Ok, So; Sun-Young, Kim; Kyu-Sik, Jo; Bo-Hee, Lee; Hee-Nam, Seo; Soo-Wan, Chae; Han-Jung, Chae.
Afiliação
  • Geum-Hwa, Lee; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Hyung-Ryong, Kim; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Sang-Yong, Han; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Bidur, Bhandary; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Do-Sung, Kim; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Min-Gul, Kim; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Byung-Ok, So; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Sun-Young, Kim; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Kyu-Sik, Jo; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Bo-Hee, Lee; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Hee-Nam, Seo; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Soo-Wan, Chae; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
  • Han-Jung, Chae; Wonkwang University. School of Dentistry. Department of Dental Pharmacology and Wonkwang Dental Research Institute. Iksan. KR
Biol. Res ; 45(4): 403-410, 2012. ilus
Artigo em Inglês | LILACS | ID: lil-668693
Biblioteca responsável: CL1.1
ABSTRACT

Objectives:

Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to β-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed.

Results:

β-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous protein (CHOP), a pro-apoptotic ER stress protein, was expressed at high levels but glucose-regulated protein 78 (GRP78), an anti-apoptotic ER stress protein with chaperone activity, was only slightly affected by treatment with β-amyloid. However, pretreatment with GE and its components inhibited the expression of CHOP but increased that of GRP78 in β-amyloid-treated cells. This study also showed that a single treatment with GE extracts, gastrodin, or 4HBA induced the expression of GRP78, a marker for enhanced protein folding machinery, suggesting a protective mechanism for GE against β-amyloid.

Conclusions:

This study reveals the protective effects of GE against β-amyloid-induced cell death, possibly through the enhancement of protein folding machinery of a representative protein, GRP78, and the regulation of CHOP in BV2 mouse microglial cells.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Álcoois Benzílicos / Sobrevivência Celular / Morte Celular / Microglia / Gastrodia / Glucosídeos / Amiloide Limite: Animais Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2012 Tipo de documento: Artigo / Documento de projeto País de afiliação: Coréia do Sul Instituição/País de afiliação: Wonkwang University/KR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Álcoois Benzílicos / Sobrevivência Celular / Morte Celular / Microglia / Gastrodia / Glucosídeos / Amiloide Limite: Animais Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2012 Tipo de documento: Artigo / Documento de projeto País de afiliação: Coréia do Sul Instituição/País de afiliação: Wonkwang University/KR
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