Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Rev. Soc. Bras. Med. Trop
; 46(5): 625-628, Sept-Oct/2013. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-691413
Biblioteca responsável:
BR1.1
ABSTRACT
Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. .
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
DNA Viral
/
Reação em Cadeia da Polimerase
/
Simplexvirus
/
Herpes Simples
/
Herpes Zoster
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Rio Grande do Sul/BR