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Gender differences in microcirculation: Observation using the hamster cheek pouch
Panazzolo, Diogo Guarnieri; da Silva, Lucia Henriques Alves; de Almeida Cyrino, Fatima Zely Garcia; Sicuro, Fernando Lencastre; Kraemer-Aguiar, Luiz Guilherme; Bouskela, Eliete.
Afiliação
  • Panazzolo, Diogo Guarnieri; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
  • da Silva, Lucia Henriques Alves; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
  • de Almeida Cyrino, Fatima Zely Garcia; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
  • Sicuro, Fernando Lencastre; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
  • Kraemer-Aguiar, Luiz Guilherme; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
  • Bouskela, Eliete; State University of Rio de Janeiro. Biomedical Center. Clinical and Experimental Research Laboratory on Vascular Biology. Rio de Janeiro. BR
Clinics ; 68(12): 1537-1542, dez. 2013. graf
Artigo em Inglês | LILACS | ID: lil-697707
Biblioteca responsável: BR1.1
ABSTRACT

OBJECTIVES:

Estrogen has been shown to play an important protective role in non-reproductive systems, such as the cardiovascular system. Our aim was to observe gender differences in vivo with regard to the increase in macromolecular permeability and leukocyte-endothelium interaction induced by ischemia/reperfusion as well as in microvascular reactivity to vasoactive substances using the hamster cheek pouch preparation.

METHODS:

Thirty-six male and 36 female hamsters, 21 weeks old, were selected for this study, and their cheek pouches were prepared for intravital microscopy. An increase in the macromolecular permeability of post-capillary venules was quantified as a leakage of intravenously injected fluorescein-labeled dextran, and the leukocyte-endothelium interaction was measured as the number of fluorescent rolling leukocytes or leukocytes adherent to the venular wall, labeled with rhodamin G, during reperfusion after 30 min of local ischemia. For microvascular reactivity, the mean internal diameter of arterioles was evaluated after the topical application of different concentrations of two vasoconstrictors, phenylephrine (α1-agonist) and endothelin-1, and two vasodilators, acetylcholine (endothelial-dependent) and sodium nitroprusside (endothelial-independent).

RESULTS:

The increase in macromolecular permeability induced by ischemia/reperfusion was significantly lower in females compared with males [19 (17-22) leaks/cm2 vs. 124 (123-128) leaks/cm2, respectively, p<0.001), but the number of rolling or adherent leukocytes was not different between the groups. Phenylephrine-induced arteriolar constriction was significantly lower in females compared with males [77 (73-102)% vs. 64 (55-69)%, p<0.04], but there were no detectable differences in endothelin-1-dependent vasoreactivity. Additionally, arteriolar vasodilatation elicited by acetylcholine or sodium nitroprusside did not differ between the groups.

CONCLUSION:

The ...
Assuntos

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Sistema Cardiovascular / Estrogênios / Microcirculação Limite: Animais Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2013 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: State University of Rio de Janeiro/BR

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Sistema Cardiovascular / Estrogênios / Microcirculação Limite: Animais Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2013 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: State University of Rio de Janeiro/BR
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