Your browser doesn't support javascript.
loading
Molecular characterization of lactobacilli isolated from fermented idli batter
Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu.
Afiliação
  • Agaliya, Perumal Jayaprabha; Pondicherry University. School of Life Sciences. Department of Biochemistry & Molecular Biology. Pondicherry. IN
  • Jeevaratnam, Kadirvelu; Pondicherry University. School of Life Sciences. Department of Biochemistry & Molecular Biology. Pondicherry. IN
Braz. j. microbiol ; 44(4): 1199-1206, Oct.-Dec. 2013. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-705261
Biblioteca responsável: BR1.1
ABSTRACT
Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Bacteriocinas / Microbiologia de Alimentos / Lactobacillus Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2013 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: Pondicherry University/IN

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Bacteriocinas / Microbiologia de Alimentos / Lactobacillus Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2013 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: Pondicherry University/IN
...