Which is the best route of administration for cell therapy in experimental model of small-for size syndrome in rats?
Acta cir. bras
; 30(2): 100-106, 02/2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-741021
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To evaluate which is the best route of administration for cell therapy in experimental rat model of small-for size syndrome.METHODS:
A total of 40 rats underwent partial hepatectomy (70%) that induces the small-for-size syndrome and were divided into four groups of route administration intravenous, intraperitoneal, enteral and tracheal. The small-for-size syndrome model was designed with extended partial hepatectomy (70%). The animals were divided into four groups of routes administration intravenous (n=10) - intravenously through the dorsal vein of the penis; intraperitoneal (n=10) - intraperitoneally in the abdominal cavity; enteral (n=10) - oroenteral with the placement of a number 4 urethral probe and maintained at third duodenal portion; tracheal (n=10) - after tracheal intubation. We track the animals and monitor them for 21 days; during this follow-up we evaluated the result of cell therapy application tracking animals using ultrasound, radiography and PET-scan. Statistical analysis was performed using GraphPad Prism Software(r). Differences were considered significant with the p<0.05. Data are presented as the median and variation for continuous variables. Comparisons between groups were made using analysis of the imaging test by the researchers.RESULTS:
All four groups underwent partial hepatectomy of 70% liver tissue targeting the same weight of resected liver. Initially the PET-scan tests showed similarity in administered cells by different routes. However, in few days the route of intravenous administration showed to be the most appropriated to lead cells to the liver followed by enteral. The tracheal and peritoneal routes were not as much successful for this goal.CONCLUSION:
The intravenous route is the best one to cell therapy in experimental rat model of small-for size-syndrome. .
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Vias de Administração de Medicamentos
/
Transplante de Células-Tronco
/
Modelos Animais de Doenças
/
Hepatopatias
/
Regeneração Hepática
Tipo de estudo:
Estudo de avaliação
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
University of Sao Paulo/BR