Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method
Braz. j. microbiol
; 45(4): 1385-1391, Oct.-Dec. 2014. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-741291
Biblioteca responsável:
BR1.1
ABSTRACT
An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Salmonella typhi
/
Febre Tifoide
/
Técnicas Bacteriológicas
/
Técnicas de Amplificação de Ácido Nucleico
/
Técnicas de Diagnóstico Molecular
Tipo de estudo:
Estudo diagnóstico
/
Estudo de avaliação
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Malásia
Instituição/País de afiliação:
Institute for Research in Molecular Medicine/MY