Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation
Braz. j. microbiol
; 45(4): 1423-1431, Oct.-Dec. 2014. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-741296
Biblioteca responsável:
BR1.1
ABSTRACT
Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride) and CV (crystal violet) application. The obtained results confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the strains did not have this ability. It was shown that P. mirabilis rods have the ability to form biofilm on the surfaces of both biomaterials applied, polystyrene and polyvinyl chloride (Nelaton catheters). The differences in ability to form biofilm observed between P. mirabilis strains derived from the urine of the catheterized and non-catheterized patients were not statistically significant.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Proteus mirabilis
/
Técnicas Microbiológicas
/
Colorimetria
/
Biofilmes
Tipo de estudo:
Pesquisa qualitativa
Limite:
Humanos
País/Região como assunto:
Europa
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Polônia
Instituição/País de afiliação:
Nicolaus Copernicus University in Torun/PL