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Biodegradation of [D-Leu1] microcystin-LR by a bacterium isolated from sediment of Patos Lagoon estuary, Brazil
Lemes, Gilmar A. F; Kist, Luiza W; Bogo, Mauricio R; Yunes, João S.
Afiliação
  • Lemes, Gilmar A. F; Universidade Federal do Rio Grande. Instituto de Oceanografia. Laboratório de Cianobactérias e Ficotoxinas. Rio Grande. BR
  • Kist, Luiza W; Pontifical Catholic University of Rio Grande do Sul. School of Biosciences. Laboratory of Genomics and Molecular Biology. Porto Alegre. BR
  • Bogo, Mauricio R; Pontifical Catholic University of Rio Grande do Sul. National Institute of Science and Technology for Translational Medicine. School of Biosciences. Porto Alegre. BR
  • Yunes, João S; Universidade Federal do Rio Grande. Instituto de Oceanografia. Laboratório de Cianobactérias e Ficotoxinas. Rio Grande. BR
J. venom. anim. toxins incl. trop. dis ; 21: 1-8, 31/03/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-741604
Biblioteca responsável: BR33.1
ABSTRACT

Background:

Toxic cyanobacterial blooms are recurrent in Patos Lagoon, in southern Brazil. Among cyanotoxins, [D-Leu1] microcystin-LR is the predominant variant whose natural cycle involves water and sediment compartments. This study aimed to identify and isolate from sediment a bacterial strain capable of growing on [D-Leu1] microcystin-LR. Sediment and water samples were collected at two distinct aquatic spots close to the Oceanographic Museum (P1), in Rio Grande City, and on São Lourenço Beach (P2), in São Lourenço do Sul City, southern Brazil.

Methods:

[D-Leu1] microcystin-LR was isolated and purified from batch cultures of Microcystis aeruginosastrain RST9501. Samples of water and sediment from Rio Grande and São Lourenço do Sul were collected. Bacteria from the samples were allowed to grow in flasks containing solely [D-Leu1] microcystin-LR. This strain named DMSX was isolated on agar MSM with 8 g L−1 glucose and further purified on a cyanotoxin basis growth. Microcystin concentration was obtained by using the ELISA immunoassay for microcystins whereas bacterial count was performed by epifluorescence microscopy. The genus Pseudomonas was identified by DNA techniques. Results; Although several bacterial strains were isolated from the samples, only one, DMXS, was capable of growing on [D-Leu1] microcystin-LR. The phylogenetic analysis of the 16S rRNA gene from DMXS strain classified the organism as Pseudomonas aeruginosa. DMXS strain incubated with [D-Leu1] microcystin-LR lowered the amount of toxin from 1 μg.L−1 to < 0.05 μg.L−1. Besides, an increase in the bacterial count–from 71 × 105 bacteria.mL−1 to 117 × 105 bacteria.mL−1–was observed along the incubation.

Conclusions:

The use of bacteria isolated from sediment for technological applications to remove toxic compounds is viable. Studies have shown that sediment plays an important role as ...
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Biodegradação Ambiental / Água / Sedimentos / Cianobactérias / Estuários / Microcistinas País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Pontifical Catholic University of Rio Grande do Sul/BR / Universidade Federal do Rio Grande/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Biodegradação Ambiental / Água / Sedimentos / Cianobactérias / Estuários / Microcistinas País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Pontifical Catholic University of Rio Grande do Sul/BR / Universidade Federal do Rio Grande/BR
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