Your browser doesn't support javascript.
loading
Control of Acid Phosphatases Expression from Aspergillus niger by Soil Characteristics
Nahas, Ely.
Afiliação
  • Nahas, Ely; Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal. Departamento de Produção Vegetal. Jaboticabal. BR
Braz. arch. biol. technol ; 58(5): 658-666, tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: lil-764475
Biblioteca responsável: BR1.1
ABSTRACT
ABSTRACTThis work studied the acid phosphatase (APase) activity from culture medium (extracellular, eAPase) and mycelial extract (intracellular, iAPase) ofAspergillus niger F111. The influence of fungus growth and phosphate concentration of the media on the synthesis and secretion of phosphatase was demonstrated. The effects of pH, substrate concentration and inorganic and organic compounds added to the reaction mixture on APase activity were also studied. Both enzymes were repressed by high concentrations of phosphate. Overexpression of iAPase in relation to eAPase was detected; iAPase activity was 46.1 times higher than eAPase. The maximal activity of eAPase was after 24h of fungus growth and for iAPase was after 96h. Optimal pH and substrate concentrations were 4.5 and 8.0 mM, respectively. Michaelis-Menten constant (Km) for the hydrolysis of p-nitrophenyl phosphate was 0.57 mM with Vmax = 14,285.71 U mg-1 mycelium for the iAPase and 0.31 mM with V max = 147.06 U mg-1 mycelium for eAPase. Organic substances had little effect on acid phosphatases when compared with the salts. Both the APases were inhibited by 10 mM KH 2PO4 and 5 mM (NH4)2MoO4; eAPase was also inhibited by 1 mM CoCl2.

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual Paulista Júlio de Mesquita Filho/BR

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual Paulista Júlio de Mesquita Filho/BR
...