Characterization of reactive stroma in prostate cancer: involvement of growth factors, metalloproteinase matrix, sexual hormones receptors and prostatic stem cells
Int. braz. j. urol
; 41(5): 849-858, Sept.-Oct. 2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-767051
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Introduction and Objectives:
Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials andMethods:
Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups Group-1 (n=20) samples without reactive stroma; Group-2 (n=20) samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens α-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Erα and ERβ.Results:
Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ERα.Conclusions:
It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Neoplasias da Próstata
/
Células-Tronco Neoplásicas
/
Adenocarcinoma
/
Células Estromais
/
Células Epiteliais
Tipo de estudo:
Estudo prognóstico
Limite:
Idoso
/
Idoso, 80 anos ou mais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Int. braz. j. urol
Assunto da revista:
Urologia
Ano de publicação:
2015
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR