Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR
Acta cir. bras
; 31(3): 206-211, Mar. 2016. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-777090
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT PURPOSE:
To determine the genetic diversity of MDR P. aeruginosa strains isolated from burn and wound infections in Ahvaz, Iran, by ERIC-PCR.METHODS:
From total 99 strains of P. aeruginosa defined as MDR by using drug susceptibility testing, 66 were subjected to ERIC-PCR analysis, comprises 53 strains isolated from burn infection, and 13 randomly selected strains from wound infection with higher resistance to combinations of more numbers of drugs.RESULTS:
Eight clusters (I to VIII), and 50 single clones were generated for tested MDR isolates analyzed by ERIC-PCR. The high heterogeneity was observed among the isolates from burn infections including 16 isolates which were categorized in eight clusters and 37 single clones. The isolates in clusters II, III, VI, VIII showed 100% similarity.CONCLUSIONS:
The high level of genotypic heterogeneity in P. aeruginosa strains demonstrated no genetic correlation between them. Extremely high drug resistance in isolates from burn, suggests that efficient control measures and proper antibiotic policy should be observed.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Pseudomonas aeruginosa
/
Infecções por Pseudomonas
/
Infecção dos Ferimentos
/
Queimaduras
/
DNA Bacteriano
/
Farmacorresistência Bacteriana Múltipla
Limite:
Humanos
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
Irã
Instituição/País de afiliação:
Ahvaz Jundishapur University of Medical Sciences/IR