Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism.
Planta Med
; 65(7): 648-50, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-10575378
ABSTRACT
DNA sequence analysis of rDNA internal transcribed spacer (ITS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were exploited for their applications in differentiating medicinal species Codonopsis pilosula, C. tangshen, C. modesta, and C. nervosa var. macrantha, from two related adulterants Campanumoea javania and Platycodon grandiflorus. The data demonstrated that the rDNA ITSI and ITSII sequences of the four Codonopsis are highly homologous but not identical, and are significantly different from those of the two adulterants. The sequence difference allows effective and reliable differentiation of Codonopsis from the adulterants by PCR-RFLP.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
RNA de Plantas
/
Asteraceae
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Planta Med
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
China