SV40, JC and BK expression in tissue, urine and blood samples from patients with malignant and nonmalignant pleural disease.
Anticancer Res
; 20(2A): 885-9, 2000.
Article
em En
| MEDLINE
| ID: mdl-10810371
ABSTRACT
BACKGROUND:
Polyomaviruses are expressed in both human tumors and immunodepressed patients. Malignant and nonmalignant pleural effusions create an environment that could favor the expression of opportunistic viral infections. We studied if SV40, JC, and BK viral DNA can be amplified from biopsies obtained from different pleural diseases. MATERIALS ANDMETHODS:
DNA was extracted from mesotheliomas (MM), nonspecific inflammatory and tubercular pleural biopsies, blood and urinary sediments from patients with MM, and pleural effusion cytological specimens. SV40, JC and BK viral early regions were amplified by PCR and analyzed by Southern Blot hybridization with specific probes.RESULTS:
SV40 was positive in 9/23 MM, 5/18 tubercular and 1/7 nonspecific inflammatory biopsies, and 5/12 pleural effusion cytological specimens. JC was positive in 2/23 MM and in 7/15 urinary sediments. All blood samples were negative and BK was also negative in all samples.CONCLUSIONS:
Tissue specific factors, characteristic of MM and TB, may contribute to expression of SV40 in these diseases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Pleurais
/
Neoplasias Pleurais
/
Vírus BK
/
Vírus JC
/
Vírus 40 dos Símios
/
Mesotelioma
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Itália