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Sequence Analysis of the Oligosaccharides from Scarlet Runner Bean Lectin.
Artigo em Inglês | MEDLINE | ID: mdl-12232596
ABSTRACT
Two oligosaccharide chains from scarlet runner bean (Phaseolus coccineus var. rubronanus) lectin (PCL) were obtained by hydrazinolysis, Bio-Gel P-2 column chromatography, NaBH(4) reduction and then separated on a Bio-Gel P-2 column. The oligosaccharides were methanolysed, acetylysed and then transformed into derivatives of trimethylsilane. Gas chromatographic studies of these two derivatives showed that their compositions were Man(8)XylGlcNAc(2) (oligosaccharide I) and Man(6)XylGlcNAc(2)Fuc (oligosaccharide II). The size of these two oligosaccharides after digestion by alpha-mannosidase, beta-xylosidase and alpha-fucosidase separately, were determined on a standardized Bio-Gel P-4 column. Thus the sequences of these two oligosaccharides were celucidated as (Manalpha)(7)ManBetaXylBeta(GlcNAc)(2) and (Manalpha)(5)ManbetaXylbeta(GlcNAc)(2)Fucalpha.

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Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) Ano de publicação: 1996 Tipo de documento: Artigo País de afiliação: China