Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints.
Syst Appl Microbiol
; 25(2): 275-83, 2002 Aug.
Article
em En
| MEDLINE
| ID: mdl-12353883
ABSTRACT
DNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67-70% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
Reação em Cadeia da Polimerase
/
Impressões Digitais de DNA
/
Pseudoalteromonas
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Syst Appl Microbiol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos