A strategy for mapping the heterochromatin of chromosome 2 of Drosophila melanogaster.
Genetica
; 117(2-3): 217-26, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12723701
ABSTRACT
The heterochromatin of chromosome 2 of Drosophila melanogaster has been among the best characterized models for functional studies of heterochromatin owing to its abundance of genetic markers. To determine whether it might also provide a favorable system for mapping extended regions of heterochromatin, we undertook a project to molecularly map the heterochromatin of the left arm of chromosome 2 (2Lh). In this paper, we describe a strategy that used clones and sequence information available from the Drosophila Genome Project and chromosome rearrangements to construct a map of the distal most portion of 2Lh. We also describe studies that used fluorescent in situ hybridization (FISH) to examine the resolution of this technique for cytologically resolving heterochromatic sequences on mitotic chromosomes. We discuss how these mapping studies can be extended to more proximal regions of the heterochromatin to determine the structural patterns and physical dimensions of 2Lh and the relationship of structure to function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Heterocromatina
/
Mapeamento Cromossômico
/
Drosophila melanogaster
Limite:
Animals
Idioma:
En
Revista:
Genetica
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos