[Polymorphism analysis of cytochrome C oxidase 1 (COI) gene in Necator americanus collected from 5 provinces in China].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 21(4): 210-3, 2003.
Article
em Zh
| MEDLINE
| ID: mdl-14628369
ABSTRACT
OBJECTIVE:
To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates.METHODS:
COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced.RESULTS:
The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation occurred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the sequences ranged from 1.34% to 2.18%.CONCLUSION:
The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Necator americanus
/
Complexo IV da Cadeia de Transporte de Elétrons
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article