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Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA.
J Sep Sci ; 28(3): 225-33, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15776923
ABSTRACT
Inexpensive and permanently modified poly(methyl methacrylate)(PMMA) microchips were fabricated by an injection-molding process. A novel sealing method for plastic microchips at room temperature was introduced. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values between 1-3% for the run-to-run and less than 2.1% for the chip-to-chip comparisons. Acrylonitrile-butadiene-styrene (ABS) was used as an additive in PMMA substrates. The proportions of PMMA and ABS were optimized. ABS may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro-osmotic flow (EOF). The detection limit of Rhodamine 6G dye for the modified microchip on the home-made microchip analyzer showed a dramatic 100-fold improvement over that for the unmodified PMMA chip. A detection limit of the order of 10(-20) mole has been achieved for each injected psiX-174/HaeIII DNA fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 DNA restriction fragments within 180 seconds. Analysis of a PCR product from the tobacco ACT gene was performed on the modified microchip as an application example.
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Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: DNA / Polimetil Metacrilato / Análise de Sequência com Séries de Oligonucleotídeos Idioma: Inglês Revista: J Sep Sci Ano de publicação: 2005 Tipo de documento: Artigo