Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana).
J Plant Physiol
; 166(4): 435-41, 2009 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-18778876
ABSTRACT
Rhodes grass (Chloris gayana) is one of the most important warm-season forage grasses. It is cultivated in tropical and subtropical parts of the world and is mostly used for grazing and hay production. We have established a particle-bombardment transformation protocol for rhodes grass using multiple-shoot clumps (MSCs) as the target tissue. A vector pAHC25 containing a herbicide-resistance gene (bar) together with the beta-glucuronidase (GUS) gene was used in transformation experiments. The most efficient recovery of bialaphos-resistant tissue was achieved when the bombarded MSCs were first cultured for 15 d on bialaphos-free medium before being subjected to selection pressure. The resistant tissues regenerated transgenic plants that displayed GUS gene expression. Under optimized conditions, 251 target pieces yielded 46 transgenic plants from 4 independent transgenic lines.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
/
Brotos de Planta
/
Biolística
/
Poaceae
Idioma:
En
Revista:
J Plant Physiol
Assunto da revista:
BOTANICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY