Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches.
J Thorac Cardiovasc Surg
; 141(4): 1056-62, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-20637475
ABSTRACT
OBJECTIVES:
Allograft vascular tissue is important in the repair of complex structural lesions of the heart and great vessels, but induces a deleterious immune response that might shorten the effective lifespan of the tissue and sensitize the recipient. We hypothesized that decellularizing allograft vascular tissue reduces the host allogeneic immune response.METHODS:
Allograft ovine pulmonary artery patches were decellularized, cryopreserved, and implanted into the descending thoracic aorta. The humoral immune response was measured by means of flow cytometry at regular intervals over 6 months. Graft histology, immunohistochemistry, and calcification were assessed after 4 weeks or 6 months.RESULTS:
Leukocyte infiltration was reduced in decellularized grafts. A trend toward decreased in-patch calcification was observed in the decellularized group (7.6 ± 4.3 vs 40.0 ± 15.9 mg of calcium/mg of protein, P = .107). Decellularization reduced IgG antibody binding to donor splenocytes (9.8% ± 3.3% vs 57.8% ± 13.7% [control value], P = .010), as assessed by means of flow cytometry. All cytokines examined were detected in nondecellularized tissues after 4 weeks but not at 6 months, indicating complete graft rejection at that time. In contrast, transforming growth factor ß1 and interleukin 10 were the only prominent cytokines in all decellularized grafts at 4 weeks after transplantation.CONCLUSIONS:
Decellularization of allograft vascular tissue minimized the recipient cellular immune response and eliminated the production of anti-donor antibodies in recipients.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artéria Pulmonar
/
Bioprótese
/
Prótese Vascular
/
Implante de Prótese Vascular
/
Tolerância ao Transplante
/
Imunidade Humoral
/
Rejeição de Enxerto
/
Sobrevivência de Enxerto
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Thorac Cardiovasc Surg
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos