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Potential utility of DNA sequence analysis of long-term-stored plant leaf fragments for forensic discrimination and identification.
Anal Sci ; 26(8): 913-6, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20702948
ABSTRACT
This study examined the potential utility of DNA sequence analysis to discriminate and identify plant material in forensic investigations. DNA was extracted from plant leaf fragments of 11 species stored for 5 to 22 years after collection. The trnH-psbA intergenic spacer and 316 bp of the rbcL gene were successfully amplified and sequenced for all fragments except for the trnH-psbA spacer of one sample. All of the plant samples were discriminated in pairwise comparisons of the sequences. Using a combination of local and global genetic databases is likely to provide greater reliability in search results to identify forensic samples from sequence data.
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Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Folhas de Planta / Genoma de Planta / DNA de Plantas / DNA Intergênico / Genética Forense Idioma: Inglês Revista: Anal Sci Ano de publicação: 2010 Tipo de documento: Artigo País de afiliação: Japão