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Quantitative high-resolution melting analysis for detecting adulterations.
Anal Biochem ; 409(1): 153-5, 2011 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-20946863
ABSTRACT
Admixtures of different plant species are a common problem in raw materials for medicinal use. Two exemplary assays were developed to admixtures in Helleborus niger with high-resolution melting analysis. HRM proved to be a very sensitive tool in detecting admixtures, able to detect a ratio of 11000 with unknown species, and of 1200,000 with Veratrum nigrum. The example proves the ability of HRM for quantification in multiplex PCR. The method is not limited to detecting adulterations. It can also be used to quantify a specific target by integrating a second amplicon in the assay as internal standard.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / DNA de Plantas Idioma: Inglês Revista: Anal Biochem Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Áustria