Taxonomic and symbiotic diversity of bacteria isolated from nodules of Acacia tortilis subsp. raddiana in arid soils of Tunisia.
Can J Microbiol
; 58(6): 738-51, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22616625
ABSTRACT
A collection of rhizobia isolated from Acacia tortilis subsp. raddiana nodules from various arid soils in Tunisia was analyzed for their diversity at both taxonomic and symbiotic levels. The isolates were found to be phenotypically diverse. The majority of the isolates tolerated 3% NaCl and grew at 40 °C. Genetic characterization emphasized that most of the strains (42/50) belong to the genus Ensifer, particularly the species Ensifer meliloti, Ensifer garamanticus, and Ensifer numidicus. Symbiotic properties of isolates showed diversity in their capacity to nodulate their host plant and to fix atmospheric nitrogen. The most effective isolates were closely related to E. garamanticus. Nodulation tests showed that 3 strains belonging to Mesorhizobium genus failed to renodulate their host plant, which is surprising for symbiotic rhizobia. Furthermore, our results support the presence of non-nodulating endophytic bacteria belonging to the Acinetobacter genus in legume nodules.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rhizobium
/
Microbiologia do Solo
/
Simbiose
/
Sinorhizobium meliloti
/
Acacia
País/Região como assunto:
Africa
Idioma:
En
Revista:
Can J Microbiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Tunísia
País de publicação:
CA
/
CANADA
/
CANADÁ