Your browser doesn't support javascript.
loading
Efficient method for Agrobacterium mediated transformation of Artemisia annua L.
Alam, Pravej; Mohammad, Anis; Ahmad, M M; Khan, Mather Ali; Nadeem, Mohd; Khan, Riyazuddeen; Akmal, Mohd; Ahlawat, Seema; Abdin, M Z.
Afiliação
  • Abdin MZ; Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, India 62. mzabdin@rediffmail.com.
Recent Pat Biotechnol ; 8(1): 102-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-22642822
ABSTRACT
Artemisinin, a potent antimalarial natural products isolated from aerial parts of Artemisia annua L. Many patents have been reported that the demand for artemisinin is exponentially increasing year after year due to increased incidences of drug resistant malaria throughout the world. Leaf explants were used frequently as target tissue to generate transgenic of Artemisia. annua L. However, obtaining a large number of transgenic lines through out the year is a laborious and delicate process. To circumvent this, we have developed a highly efficient leaf explant based Agrobacterium mediated transformation of A. annua L. plant. The gus gene was used as screenable marker to assess and optimize the performance of T-DNA delivery. The age of explant, kind of bacterial inoculation, suspension duration, infection times and co-culture conditions were optimized. The co-culture was carried out with Agrobacterium tumefaciens strain EHA105 under desiccation condition in the dark at 25-28 0C for 2-4 days. Complete analysis of transgene insertion demonstrated that the optimized method of transformation from leaf explants of A. annua L. was efficient and highly reproducible.
Assuntos
Buscar no Google
Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Neglicenciadas Tema em saúde: Malária Base de dados: MEDLINE Assunto principal: Artemisia annua / Agrobacterium Idioma: Inglês Revista: Recent Pat Biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2014 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Buscar no Google
Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Neglicenciadas Tema em saúde: Malária Base de dados: MEDLINE Assunto principal: Artemisia annua / Agrobacterium Idioma: Inglês Revista: Recent Pat Biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2014 Tipo de documento: Artigo
...