Regulation of apoptosis in human gastric cancer cell line SGC-7901 by L-arginine.
Panminerva Med
; 56(3): 227-31, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24561640
ABSTRACT
AIM:
L-arginine (L-Arg) is an aminoacid that has immunomodulating and antitumor effects. It is possible that antitumor effects of L-Arg are due to induction of apoptosis in tumor cells. The present study assessed antiproliferating and proapoptotic effects of L-Arg in human gastric cancer cell line SGC-7901.METHODS:
Cell proliferation was quantified by MTT assay. Apoptosis was assessed using flow cytometry and FITC-Annexin-V/propidium iodide staining. Expression and activation of proteins pertinent to apoptosis (Bcl-2, surviving, p53, and XIAP) were studied using PCR, Western blot, and activity assays.RESULTS:
L-Arg significantly inhibited growth of SCG-7901 gastric cancer cells and downregulated expression of antiapoptotic gene Bcl-2 and survivin. By contrast, expression of p53 was upregulated by L-Arg.CONCLUSION:
Regulation of apoptosis by L-Arg via downregulation of antiapoptotic proteins Bcl-2 and surviving, and upregulation of proapoptotic protein p53 may represent the mechanism behind antitumor effects of L-Arg.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arginina
/
Neoplasias Gástricas
/
Apoptose
Limite:
Humans
Idioma:
En
Revista:
Panminerva Med
Ano de publicação:
2014
Tipo de documento:
Article