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Optimization of preparation of antioxidative peptides from pumpkin seeds using response surface method.
PLoS One; 9(3): e92335, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24637721
ABSTRACT
Protein isolates of pumpkin (Cucurbita pepo L) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. The hydrolysis conditions were optimized through Box-Behnken experimental design combined with response surface method (RSM). The second-order model, developed for the DPPH radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). The optimal hydrolysis conditions were determined as follows: hydrolyzing temperature 50°C, pH 2.5, enzyme amount 6000 U/g, substrate concentration 0.05 g/ml and hydrolyzing time 5 h. Under the above conditions, the scavenging activity of DPPH radical was as high as 92.82%.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Peptídeos / Sementes / Biotecnologia / Cucurbita / Antioxidantes Aspecto clínico: Diagnóstico Idioma: Inglês Revista: PLoS One Assunto da revista: Ciência / Medicina Ano de publicação: 2014 Tipo de documento: Artigo País de afiliação: China