Regulation of apoptosis in human gastric cancer cell line SGC-7901 by L-arginine.
Panminerva Med
; 2014 Mar 18.
Article
em En
| MEDLINE
| ID: mdl-24642529
ABSTRACT
Aim:
L-arginine (LArg) is an amino acid that has immunomodulating and anti-tumor effects. It is possible that anti-tumor effects of L-Arg are due to induction of apoptosis in tumor cells. The present study assessed anti-proliferating and pro-apoptotic effects of L-Arg in human gastric cancer cell line SGC7901.Methods:
Cell proliferation was quantified by MTT assay. Apoptosis was assessed using flow cytometry and FITCAnnexinV/ propidium iodide staining. Expression and activation of proteins pertinent to apoptosis (Bcl2, surviving, p53, and XIAP) were studied using PCR, Western blot, and activity assays.Results:
L-Arg significantly inhibited growth of SCG-7901 gastric cancer cells and down-regulated expression of anti-apoptotic gene Bcl-2 and survivin. By contrast, expression of p53 was upregulated by L-Arg.Conclusion:
Regulation of apoptosis by L-Arg via down-regulation of anti-apoptotic proteins Bcl-2 and surviving, and up-regulation of pro-apoptotic protein p53 may represent the mechanism behind antitumor effects of L-Arg.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Panminerva Med
Ano de publicação:
2014
Tipo de documento:
Article
País de publicação:
IT
/
ITALIA
/
ITALY
/
ITÁLIA