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Two-photon multiplexing bio-imaging using a combination of Eu- and Tb-bioprobes.
Dalton Trans ; 44(11): 4918-24, 2015 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-25421299
ABSTRACT
During the course of our research studies aiming to combine the unique photophysical properties of lanthanide complexes and the intrinsic advantage of a nonlinear two-photon excitation, we described the proof-of-concept of a biphotonic multiplexing bio-imaging experiment. To that end, a new Tb-luminescent bioprobe [TbL(2)] was designed using the TACN-trispicolinate platform. The biphotonic imaging of T24 fixed cells stained either with [TbL(2)] or with the already described [EuL(1)] complexes is reported. Finally the biphotonic multiplexing experiment was performed using a combination of both probes using the spectral mode of the two-photon microscope.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Térbio / Fótons / Európio / Imagem Molecular Aspecto clínico: Prognóstico Limite: Humanos Idioma: Inglês Revista: Dalton Trans Assunto da revista: Química Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: França