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[Changes in endothelium-dependent dilation and α1-adrenoreactivity of rat aorta caused by inducible NO-synthase inhibition after motor activity restrictions].
Ross Fiziol Zh Im I M Sechenova ; 99(7): 859-68, 2013 Jul.
Article em Ru | MEDLINE | ID: mdl-25470921
ABSTRACT
The aim of work was to study the influence of the highly selective blocker of the inducible NO-synthase (iNOS) of S-methylthiourea on the alteration of the endothelium-dependent vasodilation and α1-adrenoreactivity of the isolated rat aortic rings which underwent a short-term restriction of physical activity. The experiments were carried out on rat aortic rings preparations from female-rats bathed in Krebs-Henseleit solution, bubbled with 95% O2 and 5% CO2 and contracting in isometric mode. Endothelium-dependent dilation was caused by cumulative addition of acetylcholine (10-(10)-10(-4) M) after phenylephrine precontraction(10(-6) M). Adrenoreactivity was assessed through the response to increasing concentrations of α1-adrenergic receptor agonist. The 60-minute immobilization stress, characterized by the increase of the relative weight of the adrenal glands by 19.5%, the concentration of glucocorticoids (twice as much), of NO2/NO3 (stable NO degradation products) by 35%, the reduction in the level of thyroxine (by 16%), triiodothyronine (by 10%) and the increase in thyrotropic hormone by 45%, interleukin-1b (twice as much) and the appearance of tumour necrosis factor alpha in the blood serum, was accompanied by the two types of reaction of isolated aortic rings to acetylcholine and phenylephrine. The first one was expressed in the enhancing of acetylcholine-induced dilation of isolated aortic rings and the reduction of its response to α1-adrenergic stimulant phenylephrine. The second one showed a decrease in the response of isolated aortic rings to acetylcholine and enhancing the response to phenylephrine. But both of these reaction types were eliminated by using highly selective inducible NO-synthase inhibitor with S-methylisothiourea. However, it was differently directed with a different type of reaction. Taken together, these results suggest that the iNOS is formed in the cells of rat aorta under short-term stress. In some cases it can be a source of a large number of NO (coupling state of iNOS), and in another contribute reduce its bioavailability (uncoupling state of iNOS).
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aorta / Estresse Fisiológico / Endotélio Vascular / Óxido Nítrico Sintase Tipo II Limite: Animals Idioma: Ru Revista: Ross Fiziol Zh Im I M Sechenova Assunto da revista: FISIOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de publicação: FEDERAÇÃO RUSSA / RU / RUSIA / RUSSIA
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aorta / Estresse Fisiológico / Endotélio Vascular / Óxido Nítrico Sintase Tipo II Limite: Animals Idioma: Ru Revista: Ross Fiziol Zh Im I M Sechenova Assunto da revista: FISIOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de publicação: FEDERAÇÃO RUSSA / RU / RUSIA / RUSSIA