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Fluorescent porous silicon biological probes with high quantum efficiency and stability.
Opt Express ; 22(24): 29996-30003, 2014 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-25606929
ABSTRACT
We demonstrate porous silicon biological probes as a stable and non-toxic alternative to organic dyes or cadmium-containing quantum dots for imaging and sensing applications. The fluorescent silicon quantum dots which are embedded on the porous silicon surface are passivated with carboxyl-terminated ligands through stable Si-C covalent bonds. The porous silicon bio-probes have shown photoluminescence quantum yield around 50% under near-UV excitation, with high photochemical and thermal stability. The bio-probes can be efficiently conjugated with antibodies, which is confirmed by a standard enzyme-linked immunosorbent assay (ELISA) method.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Teoria Quântica / Silício / Corantes Fluorescentes Idioma: Inglês Revista: Opt Express Assunto da revista: Oftalmologia Ano de publicação: 2014 Tipo de documento: Artigo