microRNA2993p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA.
Mol Med Rep
; 11(6): 4645-9, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25634687
ABSTRACT
Aberrant microRNA (miRNA) expression has been linked to cancer development. In this study, we aimed to investigate whether the anticancer effect of miRNA2993p on laryngeal cancer Hep2 cells is mediated through targeting human telomerase reverse transcriptase (hTERT). The expression of miR2993p in laryngeal cancer Hep2 cells and human osteosarcoma U2OS cells was quantified by stemloopmediated reverse transcription quantitative polymerase chain reaction. miR2993p mimic was transfected into Hep2 cells to induce overexpression of miR2993p. A CCK8 assay was performed to identify the effects of miR2993p overexpression on the proliferation of Hep2 cells. Western blot analysis was carried out to determine the expression of hTERT protein. A significant decrease was noted in the expression of miR2993p in Hep2 cells compared with that of U2OS cells (P<0.05). Overexpression of miR2993p resulted in a notable inhibition of cellular proliferation (P<0.05), as well as downregulation of hTERT mRNA and protein in Hep2 cells (P>0.05). The expression of miR2993p is downregulated in human laryngeal cancer Hep2 cells. Overexpression of miR2993p inhibits Hep2 cell growth by targeting the 3'untranslated region of hTERT mRNA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Telomerase
/
MicroRNAs
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
GR
/
GRECIA
/
GREECE
/
GRÉCIA