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Forensic DNA Analysis of Wheat Flour as Commonly Used in White Powder Cases.
J Forensic Sci ; 60(5): 1316-21, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26174661
ABSTRACT
In the wake of terrorist attacks using anthrax and ricin, white powder is often encountered in cases of malicious mischief and terrorist threats. Wheat flour is a common white powder encountered in such criminal investigations. We used DNA analysis to investigate wheat flour samples for identification and discrimination as trace evidence. Species identification of commercially available wheat flour was carried out by sequencing a partial region of the ribulose bisphosphate carboxylase large subunit gene (rbcL). Samples were discriminated using short tandem repeat (STR) analysis. The rbcL sequences of all wheat flour samples were identical and showed a high level of similarity to known wheat (Triticum aestivum L.) sequences. Furthermore, flours had characteristic patterns in STR analyses, with specific cultivars showing distinctive patterns. These results suggested that the identification of wheat flour species is possible using rbcL sequencing, and that STR analysis is useful for discriminating between samples.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Ribulose-Bifosfato Carboxilase / Triticum / DNA de Plantas / Farinha Limite: Humanos Idioma: Inglês Revista: J Forensic Sci Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Japão