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In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene.
Genet Mol Res ; 14(3): 7947-56, 2015 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-26214476
ABSTRACT
Tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. Previous studies have shown that Ty-2 is an important resistance gene located between molecular markers C2_At2g28250 (82.3 cM) and T0302 (89.0 cM), and exhibits strong resistance to tomato yellow leaf curl virus in Asia. In this study, Ty-2 candidate genes were subjected to bioinformatic analysis for the sequenced tomato genome. We identified 69 genes between molecular markers C2_At2g28250 and T0302, 22 of which were disease-related resistant genes, including nucleotide binding site-leucine-rich repeat disease resistance genes, protease genes (protein kinase, kinase receptor, and protein isomerase), cytochromes, and transcription factors. Expressed sequence tag analysis revealed that 77.3% (17/22) of candidate disease-resistance genes were expressed, involving 143 expressed sequence tags. Based on full-length cDNA sequence analysis, 7 candidate genes were found, 4 of which were involved in tomato responses to pathogens. Microarray expression analysis also showed that most candidate genes were involved in the tomato responses to multiple pathogens, including fungi, viruses, and bacteria. RNA-seq expression analysis revealed that all candidate genes participated in tomato growth and development.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Simulação por Computador / Mapeamento Cromossômico / Genes de Plantas / Lycopersicon esculentum / Resistência à Doença Idioma: Inglês Revista: Genet Mol Res Assunto da revista: Biologia Molecular / Genética Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: China