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Programming A Molecular Relay for Ultrasensitive Biodetection through (129)Xe NMR.
Angew Chem Int Ed Engl ; 55(5): 1733-6, 2016 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-26692420
ABSTRACT
A supramolecular strategy for detecting specific proteins in complex media by using hyperpolarized (129) Xe NMR is reported. A cucurbit[6]uril (CB[6])-based molecular relay was programmed for three sequential equilibrium conditions by designing a two-faced guest (TFG) that initially binds CB[6] and blocks the CB[6]-Xe interaction. The protein analyte recruits the TFG and frees CB[6] for Xe binding. TFGs containing CB[6]- and carbonic anhydrase II (CAII)-binding domains were synthesized in one or two steps. X-ray crystallography confirmed TFG binding to Zn(2+) in the deep CAII active-site cleft, which precludes simultaneous CB[6] binding. The molecular relay was reprogrammed to detect avidin by using a different TFG. Finally, Xe binding by CB[6] was detected in buffer and in E. coli cultures expressing CAII through ultrasensitive (129) Xe NMR spectroscopy.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Xenônio / Espectroscopia de Ressonância Magnética Idioma: Inglês Revista: Angew Chem Int Ed Engl Ano de publicação: 2016 Tipo de documento: Artigo País de afiliação: Estados Unidos