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Ultrasensitive quantitative LC-MS/MS of an inhibitor of apoptosis protein's antagonist in plasma using protein target affinity extraction.
Bioanalysis ; 8(4): 265-74, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26807991
ABSTRACT
BACKGROUND: A target protein-based affinity extraction LC-MS/MS method was developed to enable plasma level determination following ultralow dosing (0.1-3 µg/kg) of an inhibitor of apoptosis proteins molecule. Methodology & results: Affinity extraction (AE) utilizing immobilized target protein BIR2/BIR3 was used to selectively capture the inhibitor of apoptosis proteins molecule from dog plasma and enable removal of background matrix components. Pretreatment of plasma samples using protein precipitation was found to provide an additional sensitivity gain. A LLOQ of 7.8 pM was achieved by combining protein precipitation with AE. The method was used to support an ultralow dose dog toxicity study.

CONCLUSION:

AE-LC-MS/MS, utilizing target protein, is a highly sensitive methodology for small molecule quantification with potential for broader applicability.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Análise Química do Sangue / Cromatografia Líquida / Proteínas Inibidoras de Apoptose / Espectrometria de Massas em Tandem / Bibliotecas de Moléculas Pequenas / Limite de Detecção / Fracionamento Químico / Isoquinolinas Limite: Animais / Feminino / Humanos / Masculino Idioma: Inglês Revista: Bioanalysis Ano de publicação: 2016 Tipo de documento: Artigo País de afiliação: Estados Unidos