Crystal structures and mutagenesis of PPP-family ser/thr protein phosphatases elucidate the selectivity of cantharidin and novel norcantharidin-based inhibitors of PP5C.

Chattopadhyay, Debasish; Swingle, Mark R; Salter, Edward A; Wood, Eric; D'Arcy, Brandon; Zivanov, Catherine; Abney, Kevin; Musiyenko, Alla; Rusin, Scott F; Kettenbach, Arminja; Yet, Larry; Schroeder, Chad E; Golden, Jennifer E; Dunham, Wade H; Gingras, Anne-Claude; Banerjee, Surajit; Forbes, David; Wierzbicki, Andrzej; Honkanen, Richard E

Chattopadhyay, Debasish; Swingle, Mark R; Salter, Edward A; Wood, Eric; D'Arcy, Brandon; Zivanov, Catherine; Abney, Kevin; Musiyenko, Alla; Rusin, Scott F; Kettenbach, Arminja; Yet, Larry; Schroeder, Chad E; Golden, Jennifer E; Dunham, Wade H; Gingras, Anne-Claude; Banerjee, Surajit; Forbes, David; Wierzbicki, Andrzej; Honkanen, Richard E.

Afiliação

Chattopadhyay D; Department of Medicine, University of Alabama Birmingham, Birmingham, AL 35294, USA.
Swingle MR; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA.
Salter EA; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
Wood E; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
D'Arcy B; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA.
Zivanov C; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
Abney K; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA.
Musiyenko A; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA.
Rusin SF; Department of Biochemistry, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
Kettenbach A; Department of Biochemistry, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA; Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
Yet L; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
Schroeder CE; Department of Medicinal Chemistry, University of Kansas Specialized Chemistry Center, Lawrence, KS 66047, USA.
Golden JE; Department of Medicinal Chemistry, University of Kansas Specialized Chemistry Center, Lawrence, KS 66047, USA.
Dunham WH; Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Gingras AC; Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Banerjee S; Northeastern Collaborative Access Team (NE-CAT) Cornell University, Lemont, IL, USA.
Forbes D; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
Wierzbicki A; Department of Chemistry, University of South Alabama, Mobile, AL 36688, USA.
Honkanen RE; Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL 36688, USA. Electronic address: rhonkanen@southalabama.edu.

Biochem Pharmacol ; 109: 14-26, 2016 06 01.

Article em En | MEDLINE | ID: mdl-27002182

Assuntos

Compostos Bicíclicos Heterocíclicos com Pontes/química; Cantaridina/química; Inibidores Enzimáticos/química; Proteínas Nucleares/química; Fosfoproteínas Fosfatases/química; Proteína Fosfatase 1/química; Sequência de Aminoácidos; Sítios de Ligação; Domínio Catalítico; Clonagem Molecular; Cristalografia por Raios X; Escherichia coli/genética; Escherichia coli/metabolismo; Expressão Gênica; Humanos; Cinética; Simulação de Acoplamento Molecular; Mutagênese Sítio-Dirigida; Proteínas Nucleares/antagonistas & inibidores; Proteínas Nucleares/genética; Proteínas Nucleares/metabolismo; Fosfoproteínas Fosfatases/antagonistas & inibidores; Fosfoproteínas Fosfatases/genética; Fosfoproteínas Fosfatases/metabolismo; Ligação Proteica; Domínios Proteicos; Proteína Fosfatase 1/antagonistas & inibidores; Proteína Fosfatase 1/genética; Proteína Fosfatase 1/metabolismo; Estrutura Secundária de Proteína; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Alinhamento de Sequência; Relação Estrutura-Atividade

Palavras-chave

Crystal structures; Inhibitors; PP5C; Phosphatase; Screening

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cantaridina / Proteínas Nucleares / Fosfoproteínas Fosfatases / Compostos Bicíclicos Heterocíclicos com Pontes / Inibidores Enzimáticos / Proteína Fosfatase 1 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biochem Pharmacol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google