Studies on synthesis and degradation of eicosanoids by rat hepatocytes in primary culture.
J Hepatol
; 5(3): 322-31, 1987 Dec.
Article
em En
| MEDLINE
| ID: mdl-2828463
ABSTRACT
The potential of hepatocytes in primary cultures to degrade the prostanoids produced by Kupffer cells and to synthesize eicosanoids, especially leukotriene B4, after treatment with D-galactosamine was studied. Hepatocytes in primary cultures showed a substantial capability to degrade all the prostanoids produced by stimulated Kupffer cells. The rate of degradation, approx. 2 pmol/min per 10(6) hepatocytes, was nearly the same for the prostaglandins D2, E2 and F2a. Lower rates were determined for thromboxane B2 (0.4 pmol/min per 10(6) cells) and for 6-ketoprostaglandin F1a (0.2 pmol/min per 10(6) cells). The degradation products of these prostanoids lacked biological activity, e.g., reactivity with specific antibodies and the ability to contract segments of rabbit femoral artery. In the presence of 30 microM arachidonic acid, hepatocytes produced only very small amounts of prostaglandins and thromboxane, ranging from less than or equal to 22 to 50 fmol/30 min per 10(6) cells. Neither untreated nor D-galactosamine-treated hepatocytes released significant amounts of leukotriene B4. Hepatocytes appear to be the site of degradation rather than synthesis of eicosanoids in the liver.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prostaglandinas
/
Leucotrieno B4
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
J Hepatol
Assunto da revista:
GASTROENTEROLOGIA
Ano de publicação:
1987
Tipo de documento:
Article
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS