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Species identification of white false hellebore (Veratrum album subsp. oxysepalum) using real-time PCR.
Forensic Sci Int ; 275: 160-166, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28366623
ABSTRACT
Food poisoning is frequently caused by the accidental ingestion of toxic plants that possess strong morphological similarities to edible plants. False helleborine (Veratrum album) is one of the most common plants involved in such accidents. In cases of poisoning by toxic plants, rapid and accurate identification, usually based on the morphological or chemical analysis of plant parts, is required for appropriate medical treatment or forensic investigation. However, morphological examinations require experience in systematic botany because the samples are fragmentary, and chemical analysis of natural compounds can be difficult. In this study, we developed a TaqMan real-time PCR method using trnH-psbA and trnL-trnF that could be carried out in 30-60min. The lower detection limit was less than 10pg of DNA and the primer sets were specific to V. album and Veratrum stamineum. Mixed samples, cooked samples, and simulated gastric contents were successfully identified, and a multiplex assay of two regions was also possible. These results indicate that the TaqMan real-time PCR analysis is a very effective method to detect small samples of V. album and V. stamineum accurately and rapidly in poisoning cases.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Plantas Tóxicas / Veratrum / Reação em Cadeia da Polimerase em Tempo Real Limite: Humanos Idioma: Inglês Revista: Forensic Sci Int Ano de publicação: 2017 Tipo de documento: Artigo