Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4/91 by TaqMan real-time PCR.
Pol J Vet Sci
; 20(3): 599-601, 2017 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-29166280
ABSTRACT
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Bronquite Infecciosa
/
Reação em Cadeia da Polimerase em Tempo Real
Limite:
Animals
Idioma:
En
Revista:
Pol J Vet Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2017
Tipo de documento:
Article
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY