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Modeling endophilin-mediated Aß disposal in glioma cells.
Biochim Biophys Acta Mol Cell Res ; 1865(10): 1385-1396, 2018 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-30049645
Autophagy dysregulation has emerged in age-related neurological diseases (Ulland et al.; Matheoud et al.; Ashkenazi et al.). Alzheimer Disease (AD), the most common progressive neurodegenerative disorder, is characterized by the accumulation of amyloid-ß (Aß) plaques caused by aberrant Aß metabolism (Qiang et al.; Sevigny et al.; Ittner et al.). Glia constitute the brain immune system and ingest extracellular Aß for degradation via the autophagy-lysosome machinery (Ries and Sastre; Cho et al.). Here, we model the molecular rationale for this clearance process in glioma cells by showing that miR34a inhibits autophagy-mediated disposal of Aß fibrils and identifying two novel direct targets of miR34a, endophilin-3 and cathepsin B (CTSB, a previously reported enzyme for Aß degrading (Sun et al.)). Bioinformatics analyses revealed that endophilin-3 expresses at a significantly lower level in neurodegenerative diseases. Its gain-of-function substantially promotes both uptake and degradation of Aß while small interfering RNA (siRNA)-mediated endophilin-3 knockdown slowed down Aß clearance and blocked autolysosome formation. Mechanistically, gene ontology (GO) analysis of the endophilin-3 interactome identified by mass spectrometry uncovered enriched components involved in actin binding (with the highest score). Importantly, we validated that the actin-binding protein phostensin interacted with endophilin-3. Phostensin knockdown restored endophilin-3-mediated up-regulation of Aß clearance. Thus, our findings indicate that miR34a inhibits Aß clearance by targeting endophilin-3 and CTSB at multiple steps including uptake and autophagy-mediated degradation.





Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Biochim Biophys Acta Mol Cell Res Ano de publicação: 2018 Tipo de documento: Artigo