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CO2 concentration affects in vitro pig embryo developmental capacity.
Pol J Vet Sci ; 21(3): 609-614, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30468346
ABSTRACT
Culture gas atmosphere is one of the most important factors affecting embryo development in vitro. The main objective of this study was to compare the effects of CO concentration on the subsequent pre-implantation developmental capacity of pig embryos in vitro, including embryos obtained via parthenogenesis, in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI). Pig embryos were developed in four different CO2 concentrations in air: 3%, 5%, 10%, or 15%. The cleavage rate of pig parthenogenetic, IVF, or ICSI embryos developed in CO2 concen- trations under 5% was the highest. There were no significant differences in the oocyte cleavage rate in ICSI embryos in CO2 concentrations under 3% and 5% (p>0.05). However, as CO2 levels increased (up to 15%) the blastocyst output on day 7, from parthenogenetic, IVF, and ICSI em- bryos, decreased to 0%. These findings demonstrate that CO2 positively affects the developmen- tal capacity of pig embryos. However, high or low CO2 levels do not significantly improve the developmental capacity of pig embryos. The best results were obtained for all of the pig embryos at a 5% CO2 concentration.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Suínos / Dióxido de Carbono / Técnicas de Cultura Embrionária / Desenvolvimento Embrionário / Embrião de Mamíferos Limite: Animais Idioma: Inglês Revista: Pol J Vet Sci Assunto da revista: Medicina Veterinária Ano de publicação: 2018 Tipo de documento: Artigo