Your browser doesn't support javascript.
Antisense RNA Elements for Downregulating Expression.
Methods Mol Biol; 1927: 23-35, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30788783
ABSTRACT
Antisense RNA (asRNA) technology is an important tool for downregulating gene expression. When applying this strategy, the asRNA interference efficiency is determined by several elements including scaffold design, loop size, and relative abundance. Here, we take the Escherichia coli gene fabD encoding malonyl-CoA-[acyl-carrier-protein] transacylase as an example to describe the asRNA design with reliable and controllable interference efficiency. Real-time PCR and fluorescence assay methods are introduced to detect the interference efficiency at RNA level and protein level, respectively.
Assuntos

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / RNA Antissenso Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: Estados Unidos