Radioimmunoassay for detecting Mycobacterium tuberculosis antigen in cerebrospinal fluids of patients with tuberculous meningitis.
J Infect Dis
; 155(4): 608-11, 1987 Apr.
Article
em En
| MEDLINE
| ID: mdl-3102628
ABSTRACT
A biotin-avidin radioimmunoassay for detecting Mycobacterium tuberculosis antigen has been developed. The assay involves sandwiching mycobacterial antigens from sonicate preparations and cerebrospinal fluids between two antibodies produced in burros and rabbits. The reaction is amplified by using biotinylated antibody to rabbit IgG and 125I-labeled avidin. The assay has a sensitivity of 20 ng/ml and shows less than 5% cross-reactivity with six other mycobacteria. We studied patients with untreated tuberculous meningitis, patients with treated tuberculous meningitis, patients with nonbacterial meningitis, and patients with bacterial meningitis. Antigen was detectable in two of 56 control samples (40 ng/ml). Hence, samples with greater than or equal to 80 ng of antigen/ml were considered positive. In patients with untreated tuberculous meningitis, antigen levels ranged from 20 to 10,000 ng/ml, and 15 (79%) of 19 samples were positive. In the treated group, only two (10%) of 17 samples were positive. This test promises to be a rapid adjunct in the early diagnosis of tuberculous meningitis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Meníngea
/
Mycobacterium tuberculosis
/
Antígenos de Bactérias
Tipo de estudo:
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1987
Tipo de documento:
Article
País de publicação:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA