Purification, characterization and antioxidant activities of a polysaccharide from the roots of Lilium davidii var. unicolor Cotton.
Int J Biol Macromol
; 135: 1208-1216, 2019 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-31176857
ABSTRACT
A polysaccharide (LPR) was separated from the roots of Lilium davidii var. unicolor Cotton with hot water extraction, ethanol precipitation, and purification by anion-exchange and gel-permeation chromatography. LPR was characterized. The weight-average molecular weight (MW) of LPR was 5.12â¯×â¯104â¯g/moL. Glucose and mannose comprised LPR with a molar ratio of 2.93.3. IR, NMR and methylation analysis showed that LPR was a natural O-acetyl glucomannan, the backbone mainly contained ß-(1â¯ââ¯4)-linked d-glucopyranosyl and ß-(1â¯ââ¯4)-linked D-mannopyanosyl, and the branches probably linked at O-2 and/or O-3 of the mannosyl and glucosyl residues. The acetyl groups mainly attached at O-2 or O-3 of mannosyl residues. X-ray diffractometric (XRD) analysis and scanning electron microscopy (SEM) analysis revealed that LPR was a semi-crystalline substance with porous lamellar structure. Bioassays in vitro indicated that LPR had distinct scavenging activities on hydroxyl radical and DPPH radical. These findings provided a reference for functional underutilization roots of L. davidii as natural antioxidant in food and pharmaceutical industry.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Raízes de Plantas
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Lilium
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Antioxidantes
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2019
Tipo de documento:
Article