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Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay.
Methods Mol Biol; 2043: 55-62, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31463902
ABSTRACT
ADAMTS constitute a family of 19 secreted metalloproteinases involved in diverse physiopathological conditions. Most of their roles first emerged from analysis of spontaneous human and animal mutations or genetically engineered animals. However, the involved mechanisms and the full repertoire of their functions are still largely unrecognized, in part because they are difficult to produce and purify as recombinant active enzymes. Here we describe protocols, tips, and tricks specifically regarding ADAMTS2, 3, and 14 but still relevant for other ADAMTS.

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Bélgica