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Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins.
Methods Mol Biol; 2043: 93-104, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31463905
Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins.





Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Japão