Your browser doesn't support javascript.
loading
Anti-quorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1.
Parasuraman, Paramanantham; Devadatha, B; Sarma, V Venkateswara; Ranganathan, Sampathkumar; Ampasala, Dinakara Rao; Siddhardha, Busi.
Afiliação
  • Parasuraman P; Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India.
  • Devadatha B; Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, India.
  • Sarma VV; Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, India.
  • Ranganathan S; Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India.
  • Ampasala DR; Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India.
  • Siddhardha B; Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India. Electronic address: siddhardha.busi@gmail.com.
Microb Pathog ; 138: 103811, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31644930
ABSTRACT
The bacterial cell communication also termed as Quorum sensing (QS) system was involved in the expression of several virulence traits during Pseudomonas infection. The attenuating of this bacterial cell communication system is an attractive approach for the management of bacterial infections without the complication of resistance development. In this respect, the marine environment has gained significant attention due to its biodiversity and as a source of novel bioactive compounds. The present study aimed to screening effective QS inhibitors from marine associated fungal species for QS inhibitors. Twelve morphologically distinct fungal isolates were isolated from the wood of Avicennia marina from marine ecosystem. The anti-QS potential of fungal crude extract from was investigated in biosensor strain and test bacterium, Chromobacterium violaceum and Pseudomonas aeruginosa PAO1, respectively. Promising anti-QS activity was observed in the crude extract of one of the fungal isolate and identified by molecular characterization using internal transcribed spacer (ITS) region as Blastobotrys parvus PPR3. The anti-virulence and antibiofilm effects of ethyl acetate fractions from PPR3 against P. aeruginosa PAO1 were evaluated. The fungal metabolites responsible for the anti-QS activity of fungal crude extract was identified using gas chromatography-mass spectrometry (GC-MS). Furthermore, molecular docking studies were performed to understand the interaction of bioactive compounds with as receptors of P. aeruginosa PAO1. The crude extract of PPR3 showed reduction in different virulence traits of P. aeruginosa PAO1 such as production of pyocyanin, elastase, protease, chitinase, swimming and swarming motility, biofilm formation, exopolysaccharide production and alginate production at different sub-MIC concentrations. Interaction of bioactive metabolites with LasR and RhlR receptors of P. aeruginosa PAO1 was reported. The findings of the present study suggested that metabolites of B. parvus PPR3 interfere with QS system of P. aeruginosa PAO1 and alters the production of virulence factors.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Biofilmes / Saccharomycetales / Percepção de Quorum / Organismos Aquáticos / Antibiose Idioma: Inglês Revista: Microb Pathog Assunto da revista: Doenças Transmissíveis / Microbiologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Índia

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Biofilmes / Saccharomycetales / Percepção de Quorum / Organismos Aquáticos / Antibiose Idioma: Inglês Revista: Microb Pathog Assunto da revista: Doenças Transmissíveis / Microbiologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Índia
...