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High-Throughput Fluorescence Assays for Ion Channels and GPCRs.
Adv Exp Med Biol ; 1131: 27-72, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31646506
ABSTRACT
Ca2+, Na+ and K+- permeable ion channels as well as GPCRs linked to Ca2+ release are important drug targets. Accordingly, high-throughput fluorescence plate reader assays have contributed substantially to drug discovery efforts and pharmacological characterization of these receptors and ion channels. This chapter describes some of the basic properties of the fluorescent dyes facilitating these assay approaches as well as general methods for establishment and optimisation of fluorescence assays for ion channels and Gq-coupled GPCRs.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Bioensaio / Receptores Acoplados a Proteínas-G / Canais Iônicos Limite: Animais / Humanos Idioma: Inglês Revista: Adv Exp Med Biol Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Austrália