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C3N4 nanosheet-supported Prussian Blue nanoparticles as a peroxidase mimic: colorimetric enzymatic determination of lactate.
Mikrochim Acta ; 186(11): 735, 2019 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-31673799
ABSTRACT
Prussian Blue nanoparticles were deposited on g-C3N4 nanosheets. The resulting nanocomposite possesses peroxidase-like (POx) activity and can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine at room temperature in the presence of H2O2. This leads to formation of a blue product with an absorption maximum at 650 nm. The formation of the Prussian Blue nanoparticles on the g-C3N4 nanosheets, and the POx-like activity of the composite were characterized in detail. The POx mimic was used for determination of L-lactic acid via detection of H2O2 that is produced by the enzyme lactate oxidase (LOx). The assay has a linear range that extends from 5 to 100 µM, and the detection limit is 2.2 µM. The method was successfully applied to the determination of L-lactic acid in spiked human serum. Graphical abstract Ultra-small Prussian Blue (PB) nanoparticles were used to modify g-C3N4 nanosheets, and their peroxidase-like activity was explored for detection of L-lactic acid. LOx represent L-lactate oxidase, and TMB represents 3,3',5,5'-tetramethylbenzidine.

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Mikrochim Acta Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: China